Professor George Ratcliffe: List of publications

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Flux balance analysis of metabolism during growth by osmotic cell expansion and its application to tomato fruits.

Limitations of deuterium-labelled substrates for quantifying NADPH metabolism in heterotrophic Arabidopsis cell cultures

Leaf Energy Balance Requires Mitochondrial Respiration and Export of Chloroplast NADPH in the Light.

Euglena Central Metabolic Pathways and Their Subcellular Locations.

The role of nitrite and nitric oxide under low oxygen conditions in plants.

Euglena Central Metabolic Pathways and Their Subcellular Locations

Tomato roots exhibit in vivo glutamate dehydrogenase aminating capacity in response to excess ammonium supply

Computational analysis of the productivity potential of CAM

Computational analysis of the productivity potential of CAM.

Cell-Type Specific Metabolic Flux Analysis: A Challenge for Metabolic Phenotyping and a Potential Solution in Plants.

13C LABELLING FOR METABOLIC FLUX ANALYSIS IN HETEROTROPHIC EUGLENA GRACILIS

Protein-protein interactions and metabolite channelling in the plant tricarboxylic acid cycle.

Assessing Metabolic Flux in Plants with Radiorespirometry.

Nitrite Protects Mitochondrial Structure and Function under Hypoxia.

Plant Science Applications of NMR

GABA (γ-Aminobutyric Acid) Uptake Via the GABA Permease GabP Represses Virulence Gene Expression in Pseudomonas syringae pv. tomato DC3000.

Lipogenesis and Redox Balance in Nitrogen-Fixing Pea Bacteroids.

Plant science applications of NMR

A Method of Accounting for Enzyme Costs in Flux Balance Analysis Reveals Alternative Pathways and Metabolite Stores in an Illuminated Arabidopsis Leaf.

Nitric oxide induces the alternative oxidase pathway in Arabidopsis seedlings deprived of inorganic phosphate.

Fluxes through plant metabolic networks: measurements, predictions, insights and challenges.

Plant mitochondria: Source and target for nitric oxide

Plant mitochondria: source and target for nitric oxide.

Functional roles of nitric oxide in roots and mitochondria

Quantification of13C enrichments and isotopomer abundances for metabolic flux analysis using 1D NMR spectroscopy.

Guarding the guard cells?

A Diel Flux Balance Model Captures Interactions between Light and Dark Metabolism during Day-Night Cycles in C3 and Crassulacean Acid Metabolism Leaves.

The metabolic flux phenotype of heterotrophic Arabidopsis cells reveals a flexible balance between the cytosolic and plastidic contributions to carbohydrate oxidation in response to phosphate limitation.

Editorial overview: Synthetic plant biology: the roots of a bio-based society

Editorial overview: Synthetic plant biology: the roots of a bio-based society.

Nitric oxide is required for homeostasis of oxygen and reactive oxygen species in barley roots under aerobic conditions.

Metabolic flux phenotype of tobacco hairy roots engineered for increased geraniol production

Metabolic flux phenotype of tobacco hairy roots engineered for increased geraniol production.

Optimization of steady-state 13C-labeling experiments for metabolic flux analysis

Optimization of steady-state ¹³C-labeling experiments for metabolic flux analysis.

Quantification of ¹³C enrichments and isotopomer abundances for metabolic flux analysis using 1D NMR spectroscopy.

A method for accounting for maintenance costs in flux balance analysis improves the prediction of plant cell metabolic phenotypes under stress conditions

A method for accounting for maintenance costs in flux balance analysis improves the prediction of plant cell metabolic phenotypes under stress conditions.

Modelling metabolic CO2 evolution - A fresh perspective on respiration

Modelling metabolic CO₂ evolution--a fresh perspective on respiration.

The metabolic flux phenotype of heterotrophic Arabidopsis cells reveals a complex response to changes in nitrogen supply

The metabolic flux phenotype of heterotrophic Arabidopsis cells reveals a complex response to changes in nitrogen supply.

Characterization of ammonium and nitrate uptake and assimilation in roots of tea plants

Metabolic engineering of tomato fruit organic acid content guided by biochemical analysis of an introgression line.

Mitochondrial cysteine synthase complex regulates O-acetylserine biosynthesis in plants.

Pathways and fluxes: exploring the plant metabolic network.

Strategies for investigating the plant metabolic network with steady-state metabolic flux analysis: lessons from an Arabidopsis cell culture and other systems.

Capturing metabolite channeling in metabolic flux phenotypes.

13c metabolic flux analysis identifies an unusual route for pyruvate dissimilation in mycobacteria which requires isocitrate lyase and carbon dioxide fixation

Deamination role of inducible glutamate dehydrogenase isoenzyme 7 in Brassica napus leaf protoplasts

Flux-balance modeling of plant metabolism.

Plant Science Applications of NMR

A genome-scale metabolic model accurately predicts fluxes in central carbon metabolism under stress conditions.

Not just a circle: flux modes in the plant TCA cycle.

Subcellular flux analysis of central metabolism in a heterotrophic Arabidopsis cell suspension using steady-state stable isotope labeling.

Characterization of glycolytic initial metabolites and enzyme activities in developing sunflower (Helianthus annuus L.) seeds.

Insights into plant metabolic networks from steady-state metabolic flux analysis.

Insights into plant metabolic networks from steady-state metabolic flux analysis.

Quantification of Isotope Label

Response of cytoplasmic pH to anoxia in plant tissues with altered activities of fermentation enzymes: application of methyl phosphonate as an NMR pH probe.

Metabolic network fluxes in heterotrophic Arabidopsis cells: stability of the flux distribution under different oxygenation conditions.

Decrease in manganese superoxide dismutase leads to reduced root growth and affects tricarboxylic acid cycle flux and mitochondrial redox homeostasis.

1H NMR metabolite fingerprinting and metabolomic analysis of perchloric acid extracts from plant tissues.

Metabolic Organization in Plants: A Challenge for the Metabolic Engineer

Glycolytic enzymes associate dynamically with mitochondria in response to respiratory demand and support substrate channeling.

Dynamic metabolic networks: Going with the flow

Network flux analysis: impact of 13C-substrates on metabolism in Arabidopsis thaliana cell suspension cultures.

Vacuolar compartmentation complicates the steady-state analysis of glucose metabolism and forces reappraisal of sucrose cycling in plants.

Deficiency of mitochondrial fumarase activity in tomato plants impairs photosynthesis via an effect on stomatal function.

Nitrite reduces cytoplasmic acidosis under anoxia.

The response of carbohydrate metabolism in potato tubers to low temperature

The response of carbohydrate metabolism in potato tubers to low temperature.

Measuring multiple fluxes through plant metabolic networks.

Physiological and metabolic adaptations of Potamogeton pectinatus L. tubers support rapid elongation of stem tissue in the absence of oxygen.

Microelectrode and 133Cs nuclear magnetic resonance evidence for variable cytosolic and cytoplasmic nitrate pools in maize root tips

Enhanced photosynthetic performance and growth as a consequence of decreasing mitochondrial malate dehydrogenase activity in transgenic tomato plants.

NMR analysis of plant nitrogen metabolism

Revealing metabolic phenotypes in plants: inputs from NMR analysis.

Metabolite fingerprinting and profiling in plants using NMR.

Activation and function of mitochondrial uncoupling protein in plants.

Quantitative approaches for analysing fluxes through plant metabolic networks using NMR and stable isotope labelling

The role of nitrate reduction in the anoxic metabolism of roots II. Anoxic metabolism of tobacco roots with or without nitrate reductase activity

Evidence for the involvement of tetrahydrofolate in the demethylation of nicotine by Nicotiana plumbaginifolia cell-suspension cultures

Activation of pyrophosphate:fructose-6-phosphate 1-phosphotransferase by fructose 2,6-bisphosphate stimulates conversion of hexose phosphates to triose phosphates but does not influence accumulation of carbohydrates in phosphate-deficient tobacco cells.

Abscisic acid concentration, root pH and anatomy do not explain growth differences of chickpea (Cicer arietinum L.) and lupin (Lupinus angustifolius L.) on acid and alkaline soils

Utilization of glycine and serine as nitrogen sources in the roots of Zea mays and Chamaegigas intrepidus

Plant NMR spectroscopy

PROBING PLANT METABOLISM WITH NMR.

Contribution of glutamate dehydrogenase to mitochondrial glutamate metabolism studied by 13 C and 31 P nuclear magnetic resonance

Contribution of glutamate dehydrogenase to mitochondrial glutamate metabolism studied by 13C and 31P nuclear magnetic resonance

Fructose 2,6-bisphosphate activates pyrophosphate: fructose-6-phosphate 1-phosphotransferase and increases triose phosphate to hexose phosphate cycling in heterotrophic cells.

31P NMR for the study of P metabolism and translocation in arbuscular mycorrhizal fungi

Anoxia tolerance in the aquatic monocot Potamogeton pectinatus : absence of oxygen stimulates elongation in association with an unusually large Pasteur effect

An in vivo 15N NMR study of agropine synthesis in transformed root cultures of Nicotiana tabacum

Urea: a nitrogen source for the aquatic resurrection plant Chamaegigas intrepidus Dinter

Strategies for metabolic flux analysis in plants using isotope labelling.

Anoxia tolerance in the aquatic monocot Potamogeton pectinatus: Absence of oxygen stimulates elongation in association with an unusually large Pasteur effect

Intracellular pH regulation in maize root tips exposed to ammonium at high external pH

Lactate metabolism in potato tubers deficient in lactate dehydrogenase activity

Prospects for in vivo NMR methods in xenobiotic research in plants

In vivo nuclear-magnetic-resonance analysis of polyamine and alkaloid metabolism in transformed root cultures of Datura stramonium L.: Evidence for the involvement of putrescine in phytohormone-induced de-differentiation

Intracellular pH stability in the aquatic resurrection plant Chamaegigas intrepidus in the extreme environmental conditions that characterize its natural habitat

Physiological and biochemical processes related to ammonium toxicity in higher plants

Ammonium assimilation in bryophytes. L-glutamine synthetase from Sphagnum fallax

An in vivo 31P NMR study of the phosphorus metabolites in developing seeds of wheat, soybean and mustard

An investigation of N metabolism and pH regulation in Sphagnum using in vivo nuclear magnetic resonance and stable isotope mass spectrometry

In vivo nmr studies of the metabolic response of plant tissues to anoxia

Using Bulk Magnetic Susceptibility to Resolve Internal and External Signals in the NMR Spectra of Plant Tissues

Phytohormone-induced GABA production in transformed root cultures of Datura stramonium: An in vivo 15N NMR study

Measuring nitrate in plant cells by in vivo NMR using Gd3+ as a shift reagent

In vivo NMR analysis of tropane alkaloid metabolism in transformed root and de-differentiated cultures of Datura stramonium

THE INFLUENCE OF NITROGEN AND POTASSIUM SUPPLY ON THE AMMONIUM CONTENT OF MAIZE (ZEA-MAYS L) LEAVES INCLUDING A COMPARISON OF MEASUREMENTS MADE IN-VIVO AND IN-VITRO

PARTITIONING OF INTERMEDIARY CARBON METABOLISM IN VESICULAR-ARBUSCULAR MYCORRHIZAL LEEK

Evidence for deamination by glutamate dehydrogenase in higher plants: Commentary

Manipulating cytoplasmic pH under anoxia: A critical test of the role of pH in the switch from aerobic to anaerobic metabolism

Mechanisms and consequences of protein adsorption on soil mineral surfaces

METABOLIC ASPECTS OF THE ANOXIC RESPONSE IN PLANT TISSUE

Effects of vanadate on the ATP content, ATPase activity and phosphate absorption capacity of maize roots

Ammonium assimilation and the role of gamma-aminobutyric acid in pH homeostasis in carrot cell suspensions

In vivo NMR Studies of Higher Plants and Algae

In vivo 15N NMR studies of secondary metabolism in transformed root cultures of Datura stramonium and Nicotiana tabacum

A 1P-NMR study of the uptake and compartmentation of manganese by maize roots

Quantitative aspects of the 1P-NMR detection of manganese in plant tissues12

Nuclear magnetic resonance studies of the location and function of plant nutrients in vivo

Relationship between intracellular pH and N metabolism in maize (Zea mays L.) roots

Interpretation of the pH dependence of protein adsorption on clay mineral surfaces and its relevance to the understanding of extracellular enzyme activity in soil

Metabolic studies of a fructose-intolerant yeast by in vivo 31P-nuclear magnetic resonance spectroscopy.

An in Vivo Nuclear Magnetic Resonance Investigation of Ion Transport in Maize (Zea mays) and Spartina anglica Roots during Exposure to High Salt Concentrations.

Subcellular distribution of inorganic phosphate, and levels of nucleoside triphosphate, in mature maize roots at low external phosphate concentrations: Measurements with 31P-NMR

OBSERVATIONS ON THE SUBCELLULAR-DISTRIBUTION OF THE AMMONIUM ION IN MAIZE ROOT-TISSUE USING INVIVO N-14-NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY (PLANTA, VOL 183, PG 359, 1991)

Nitrogen assimilation and the control of ammonium and nitrate absorption by maize roots

Metabolic Response of Maize Roots to Hyperosmotic Shock : An in VivoP Nuclear Magnetic Resonance Study.

DIRECT OBSERVATION OF THE EFFECTS OF 3,5-DIISOPROPYLSALICYLATE AND LIGHT ON MAIZE COLEOPTILES USING P-31 NMR

AVOIDANCE OF HYPOXIA IN A CNIDARIAN SYMBIOSIS BY ALGAL PHOTOSYNTHETIC OXYGEN

A 31P NMR study of the adsorption of bovine serum albumin on montmorillonite using phosphate and the paramagnetic cation Mn2+: modification of conformation with pH

Detection of 15N-labeled ammonium. In Vivo, 15N NMR versus mass spectrometry

Observations on the subcellular distribution of the ammonium ion in maize root tissue using in-vivo 14N-nuclear magnetic resonance spectroscopy

The role of glutamate dehydrogenase in plant nitrogen metabolism

31P nuclear magnetic resonance evidence for differences in intracellular pH in the roots of maize seedlings grown with nitrate or ammonium

P-31 NUCLEAR-MAGNETIC-RESONANCE EVIDENCE FOR DIFFERENCES IN INTRACELLULAR PH IN THE ROOTS OF MAIZE SEEDLINGS GROWN WITH NITRATE OR AMMONIUM

31P NMR measurements of the cytoplasmic and vacuolar Pi content of mature maize roots: Relationships with phosphorus status and phosphate fluxes

H-1 AND P-31 NMR INVESTIGATION OF GADOLINIUM UPTAKE IN MAIZE ROOTS

1H and 31P NMR investigation of gadolinium uptake in maize roots

31P NMR observations on the effect of the external pH on the intracellular pH values in plant cell suspension cultures

Applications of NMR in the Analysis of Agrochemicals and Pesticides

A C-13 NMR-STUDY OF PHOTOSYNTHATE TRANSPORT AND METABOLISM IN THE LICHEN XANTHORIA-CALCICOLA OXNER

Observations on the cytoplasmic and vacuolar orthophosphate pools in leaf tissues using in vivo 31P-NMR spectroscopy

Airlift systems for in vivo NMR spectroscopy of plant tissues

Galactose metabolism in Zea mays root tissues observed by 31P-NMR spectroscopy

Prospects for NMR imaging in the study of biological morphogenesis.

THE PHYSIOLOGY OF BASIDIOMYCETE LINEAR ORGANS .2. PHOSPHATE-UPTAKE BY RHIZOMORPHS OF ARMILLARIA-MELLEA

Phosphorus metabolism and intracellular pH in the halotolerant alga Dunaliella parva studied by 31P-NMR

High resolution imaging of plant tissues10

HIGH-RESOLUTION IMAGING OF PLANT-TISSUES BY NMR

APPLICATION OF NUCLEAR MAGNETIC-RESONANCE METHODS TO PLANT-TISSUES

NMR and the inorganic composition of plants

31P NMR measurements of cytoplasmic ph changes in maize root tips

A 14N nuclear magnetic resonance study of inorganic nitrogen métabolism in barley, maize and pea roots

13C NMR determination of sugar levels in storage roots of carrot (Daucus carota)

NMR and compartmentation in biological tissues

Intracellular distribution of phosphate in cultured Humulus lupulus cells growing at elevated exogenous phosphate concentrations.

An investigation of the growth characteristics of oil-palm (Elaeis guineensis) suspension cultures using 31P NMR.

Development of an aeration system for use in plant tissue 9

Phosphorus nutrition and the intracellular distribution of inorganic phosphate in pea root tips: A quantitative study using31P-NMR

DEVELOPMENT OF AN AERATION SYSTEM FOR USE IN PLANT-TISSUE NMR EXPERIMENTS

NMR AND THE BIOCHEMIST

PRECIPITATION WITHIN UNILAMELLAR VESICLES .2. MEMBRANE CONTROL OF ION-TRANSPORT

The application of 31P nuclear magnetic resonance to higher plant tissue: I. Detection of spectra

The application of 31P nuclear magnetic resonance to higher plant tissue: II. Detection of intracellular changes

TIME DEVELOPMENT OF PROTON NUCLEAR OVERHAUSER EFFECTS IN PROTEINS

A Proton NMR Study of Ribosomal Protein L25 from Escherichia coli

ON THE RENATURATION OF RIBOSOMAL PROTEIN-L11

TRANSVERSE RELAXATION EFFECTS RESULTING FROM DEUTERIUM-EXCHANGE IN THE PROTON NMR-SPECTRA OF PROTEINS

Nuclear-magnetic-resonance studies of 5'-ribonucleotide and 5'-deoxyribonucleotide conformations in solution using the lanthanide probe method.

FOURIER-TRANSFORM NMR PULSE METHODS FOR MEASUREMENT OF SLOW-EXCHANGE RATES

METHOD FOR ACCURATE MEASUREMENT OF H-1 SPIN-SPIN COUPLING-CONSTANTS IN LARGE MOLECULES

FOURIER-TRANSFORM PROTON NMR IN H2O - METHOD FOR MEASURING EXCHANGE AND RELAXATION RATES

Structure of Gene 5 Protein-Oligodeoxynucleotide Complexes as Determined by 1H, 19F, and 31P Nuclear Magnetic Resonance